A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection.
Dung, Tran Thi Ngoc;
Duy, Pham Thanh;
Sessions, October M;
Sangumathi, Uma K;
Phat, Voong Vinh;
Tam, Pham Thi Thanh;
To, Nguyen Thi Nguyen;
Phuc, Tran My;
Hong Chau, Tran Thi;
Chau, Nguyen Ngoc Minh;
+4 more...Minh, Ngoc Nguyen;
Thwaites, Guy E;
Rabaa, Maia A;
Baker, Stephen;
(2017)
A universal genome sequencing method for rotavirus A from human fecal samples which identifies segment reassortment and multi-genotype mixed infection.
BMC genomics, 18 (1).
324-.
ISSN 1471-2164
DOI: https://doi.org/10.1186/s12864-017-3714-6
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BACKGROUND: Genomic characterization of rotavirus (RoV) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material. METHODS: To overcome these limitations, we developed a novel RoV capture and genome sequencing method combining commercial enzyme immunoassay plates and a set of routinely used reagents. RESULTS: Our approach had a 100% success rate, producing >90% genome coverage for diverse RoV present in fecal samples (Ct < 30). CONCLUSIONS: This method provides a novel, reproducible and comparatively simple approach for genomic RoV characterization and could be scaled-up for use in global RoV surveillance systems. TRIAL REGISTRATION (PROSPECTIVELY REGISTERED): Current Controlled Trials ISRCTN88101063 . Date of registration: 14/06/2012.