Etiology of severe childhood pneumonia in the Gambia, West Africa, determined by conventional and molecular microbiological analyses of lung and pleural aspirate samples.

Howie, Stephen RC; Morris, Gerard AJ; Tokarz, Rafal; Ebruke, Bernard E; Machuka, Eunice M; Ideh, Readon C; Chimah, Osaretin; Secka, Ousman; Townend, John; Dione, Michel; +11 more...Oluwalana, Claire; Njie, Malick; Jallow, Mariatou; Hill, Philip C; Antonio, MartinORCID logo; Greenwood, BrianORCID logo; Briese, Thomas; Mulholland, KimORCID logo; Corrah, Tumani; Lipkin, W Ian; and Adegbola, Richard A (2014) Etiology of severe childhood pneumonia in the Gambia, West Africa, determined by conventional and molecular microbiological analyses of lung and pleural aspirate samples. Clinical infectious diseases, 59 (5). pp. 682-685. ISSN 1058-4838 DOI: 10.1093/cid/ciu384
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Molecular analyses of lung aspirates from Gambian children with severe pneumonia detected pathogens more frequently than did culture and showed a predominance of bacteria, principally Streptococcus pneumoniae, >75% being of serotypes covered by current pneumococcal conjugate vaccines. Multiple pathogens were detected frequently, notably Haemophilus influenzae (mostly nontypeable) together with S. pneumoniae.


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