Eicosapentaenoic acid and 3,10 dithia stearic acid inhibit the desaturation of trans-vaccenic acid into cis-9, trans-11-conjugated linoleic acid through different pathways in Caco-2 and T84 cells


Renaville, B; Mullen, A; Moloney, F; Larondelle, Y; Schneider, YJ; Roche, HM; (2006) Eicosapentaenoic acid and 3,10 dithia stearic acid inhibit the desaturation of trans-vaccenic acid into cis-9, trans-11-conjugated linoleic acid through different pathways in Caco-2 and T84 cells. The British journal of nutrition, 95 (4). pp. 688-95. ISSN 0007-1145 DOI: https://doi.org/10.1079/BJN20061717

[img]
Preview
Text - Published Version
License:

Download (131kB) | Preview

Abstract

Stearoyl-CoA desaturase (SCD) is a key enzyme that determines the composition and metabolic fate of ingested fatty acids, in particular the conversion of trans-vaccenic acid (TVA) to conjugated linoleic acid (CLA). The present study addressed the hypothesis that intestinal TVA absorption and biotransformation into CLA can be modulated by EPA and 3,10-dithia stearic acid (DSA) via altered SCD mRNA levels and desaturation indices (cis-9, trans-11-CLA:TVA and oleic acid:stearic acid ratios) in Caco-2 and T84 cells, two well-established in vitro models of the human intestinal epithelium. The study determined the effect of acute (3 h with 0.3 mm-EPA or 0.3 mm-DSA) and acute-on-chronic (1 week with 0.03 mm-EPA or -DSA, followed by respectively, 0.3 mm-EPA or -DSA for 3 h) treatments. In both cell lines, acute EPA treatment did not alter SCD desaturation indices, whereas the acute-on-chronic treatment affected these surrogate markers of SCD activity. This was associated with reduced sterol regulatory-element binding protein-1c and SCD mRNA levels. In contrast, acute and acute-on-chronic DSA treatments significantly reduced SCD desaturation indices without affecting SCD mRNA levels in Caco-2 cells. The present study on intestinal cells shows that the conversion rate of TVA to c9, t11-CLA is affected by other fatty acids present in the diet such as EPA, confirming previous observations in hepatic and mammary cell models.

Item Type: Article
Keywords: Caco-2 Cells, Cell Line, Cell Proliferation/drug effects, Drug Administration Schedule, Eicosapentaenoic Acid/*pharmacology, Epithelial Cells/metabolism, Gene Expression Regulation, Enzymologic/drug effects, Humans, Intestinal Mucosa/*drug effects/metabolism, Linoleic Acids, Conjugated/*metabolism, Oleic Acids/*metabolism, RNA, Messenger/genetics, Stearic Acids/*pharmacology, Stearoyl-CoA Desaturase/antagonists & inhibitors/biosynthesis/genetics, Caco-2 Cells, Cell Line, Cell Proliferation, drug effects, Drug Administration Schedule, Eicosapentaenoic Acid, pharmacology, Epithelial Cells, metabolism, Gene Expression Regulation, Enzymologic, drug effects, Humans, Intestinal Mucosa, drug effects, metabolism, Linoleic Acids, Conjugated, metabolism, Oleic Acids, metabolism, RNA, Messenger, genetics, Stearic Acids, pharmacology, Stearoyl-CoA Desaturase, antagonists & inhibitors, biosynthesis, genetics
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Population Health (2012- ) > Dept of Nutrition and Public Health Interventions Research (2003-2012)
Faculty of Epidemiology and Population Health > Dept of Population Health (2012- )
PubMed ID: 16571147
Web of Science ID: 236386600003
URI: http://researchonline.lshtm.ac.uk/id/eprint/8992

Statistics


Download activity - last 12 months
Downloads since deposit
260Downloads
343Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item