Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions


Antonio, M; Hakeem, I; Sankareh, K; Cheung, YB; Adegbola, RA; (2009) Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions. Journal of medical microbiology, 58 (3). pp. 296-302. ISSN 0022-2615 DOI: https://doi.org/10.1099/jmm.0.006031-0

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Abstract

Sequential multiplex PCR was evaluated for detection of multiple Streptococcus pneumoniae serotypes directly from nasopharyngeal secretions. A total of 279 nasopharyngeal swab samples were tested blindly. When limited to the 29 serotypes identifiable by the molecular method, the mean number of serotypes identified by the conventional latex/Quellung method was 0.85, which was significantly lower than that by the molecular method (P < 0.0001). The multiplex PCR method identified significantly more serotypes than the latex/Quellung method if limited to the 29 serotypes (P = 0.001 and P = 0.014, respectively).

Item Type: Article
Keywords: Binomial Distribution, Carrier State, microbiology, Cross-Sectional Studies, DNA, Bacterial, isolation & purification, Gambia, Humans, Latex Fixation Tests, Nasopharynx, microbiology, Pneumococcal Infections, microbiology, Pneumococcal Vaccines, standards, Polymerase Chain Reaction, methods, Probability, Sensitivity and Specificity, Serotyping, Streptococcus pneumoniae, classification, genetics, isolation & purification, Vaccines, Conjugate, standards
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
PubMed ID: 19208877
Web of Science ID: 263921600003
URI: http://researchonline.lshtm.ac.uk/id/eprint/5594

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