Genome-based analysis of Enterococcus faecium bacteremia associated with recurrent and mixed strain infection.

Raven, KE; Gouliouris, T; Parkhill, J; Peacock, SJ; (2017) Genome-based analysis of Enterococcus faecium bacteremia associated with recurrent and mixed strain infection. Journal of clinical microbiology. ISSN 0095-1137 DOI:

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Vancomycin-resistant Enterococcus faecium (VREfm) bloodstream infections are associated with high recurrence rates. This study used genome sequencing to accurately distinguish the frequency of relapse and reinfection in patients with recurrent E. faecium bacteremia, and to investigate strain relatedness in patients with apparent VREfm and vancomycin-susceptible E. faecium (VSEfm) mixed infection. A retrospective study was performed at the Cambridge University Hospitals NHS Foundation Trust (CUH) between November 2006 and December 2012. We analyzed the genomes of 44 E. faecium isolated from 21 patients (26 VREfm from 12 patients with recurrent bacteremia, and 18 isolates from 9 patients with putative VREfm/VSEfm mixed infection). Phenotypic antibiotic susceptibility was determined using the Vitek2 instrument. Genomes were compared with those for a further 263 E. faecium associated with bacteremia in patients at CUH over the same time period. Pairwise comparison of core genomes indicated that 10 (71%) episodes of recurrent VREfm bacteremia were due to reinfection with a new strain, with reinfection being more likely with increasing time between the two positive cultures. The majority (78%) of patients with a mixed VREfm and VSEfm infection had unrelated strains. More than half (59%) of study isolates were closely related to another isolate associated with bacteremia from CUH. This included 60% of isolates associated with re-infection, indicating acquisition in the hospital. This study provides the first high-resolution insights into recurrence and mixed infection by E. faecium, and demonstrates that reinfection with a new strain, often acquired from the hospital, is a driver of recurrence.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Antimicrobial Resistance Centre (AMR)
PubMed ID: 29263205


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