Functional analysis of leucine aminopeptidase in Caenorhabditis elegans


Joshua, GW; (2001) Functional analysis of leucine aminopeptidase in Caenorhabditis elegans. Molecular and biochemical parasitology, 113 (2). pp. 223-32. ISSN 0166-6851

Full text not available from this repository. (Request a copy)

Abstract

To investigate the function of the enzyme leucine aminopeptidase in nematodes, a Caenorhabditis elegans leucine aminopeptidase gene identified in the genome sequence was functionally analysed by transfection of a leucine aminopeptidase beta-galactosidase reporter construct and characterisation of a null mutant. The leucine aminopeptidase transgene is expressed along the length of the gut, and immunolocalisation shows the enzyme in the buccal cavity, pharynx, anterior gut and rectum. It is constitutively expressed as seen by analysis of cDNAs constructed from mRNAs of nematodes taken at 2 h intervals through the life-cycle; and by western blot analysis of protein from the same set of nematodes. Leucine aminopeptidase null mutants had a slower growth rate and delayed onset of egg-laying. We suggest that in C. elegans, leucine aminopeptidase is a digestive enzyme.

Item Type: Article
Keywords: Amino Acid Sequence, Animal, Caenorhabditis elegans/*enzymology/genetics, Gene Deletion, Immunohistochemistry, Lac Operon/genetics, Leucyl Aminopeptidase/chemistry/*genetics/*metabolism, Molecular Sequence Data, Recombinant Fusion Proteins/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Support, Non-U.S. Gov't, Transfection, Transgenes, Amino Acid Sequence, Animal, Caenorhabditis elegans, enzymology, genetics, Gene Deletion, Immunohistochemistry, Lac Operon, genetics, Leucyl Aminopeptidase, chemistry, genetics, metabolism, Molecular Sequence Data, Recombinant Fusion Proteins, metabolism, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Support, Non-U.S. Gov't, Transfection, Transgenes
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 11295176
Web of Science ID: 168308300004
URI: http://researchonline.lshtm.ac.uk/id/eprint/17583

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
309Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item