Bromelain activates murine macrophages and natural killer cells in vitro


Engwerda, CR; Andrew, D; Murphy, M; Mynott, TL; (2001) Bromelain activates murine macrophages and natural killer cells in vitro. Cellular immunology, 210 (1). pp. 5-10. ISSN 0008-8749 DOI: https://doi.org/10.1006/cimm.2001.1793

Full text not available from this repository.

Abstract

The innate immune response is critical for effective immunity against most pathogens. In this study, we show that bromelain, a mixture of cysteine proteases, can enhance IFN-gamma-mediated nitric oxide and TNFalpha production by macrophages. Bromelain's effect was independent of endotoxin receptor activation and was not caused by direct modulation of IFN-gamma receptors. Instead, bromelain either enhanced or acted synergistically with IFN-gamma receptor-mediated signals. These effects were seen in both RAW 264.7, a macrophage cell line, and primary macrophage populations. Bromelain also increased IL-2- and IL-12-mediated IFN-gamma production by NK cells. These results indicate a potential role for bromelain in the activation of inflammatory responses in situations where they may be deficient, such as may occur in immunocompromised individuals.

Item Type: Article
Keywords: Animal, Bromelains/*pharmacology, Cell Line, Cells, Cultured, Drug Synergism, Female, Interferon Type II/metabolism/pharmacology, Killer Cells, Natural/drug effects/*immunology, Macrophage Activation/drug effects, Macrophages/drug effects/*immunology, Mice, Mice, Inbred BALB C, Nitric Oxide/biosynthesis, Receptors, Interferon/metabolism, Support, Non-U.S. Gov't, Tumor Necrosis Factor/biosynthesis, Animal, Bromelains, pharmacology, Cell Line, Cells, Cultured, Drug Synergism, Female, Interferon Type II, metabolism, pharmacology, Killer Cells, Natural, drug effects, immunology, Macrophage Activation, drug effects, Macrophages, drug effects, immunology, Mice, Mice, Inbred BALB C, Nitric Oxide, biosynthesis, Receptors, Interferon, metabolism, Support, Non-U.S. Gov't, Tumor Necrosis Factor, biosynthesis
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
PubMed ID: 11485347
Web of Science ID: 170498200002
URI: http://researchonline.lshtm.ac.uk/id/eprint/17129

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
352Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item