Comparative loss and maturation of peripheral blood dendritic cell subpopulations in African and non-African HIV-1-infected patients


Jones, GJ; Watera, C; Patterson, S; Rutebemberwa, A; Kaleebu, P; Whitworth, JA; Gotch, FM; Gilmour, JW; (2001) Comparative loss and maturation of peripheral blood dendritic cell subpopulations in African and non-African HIV-1-infected patients. AIDS (London, England), 15 (13). pp. 1657-63. ISSN 0269-9370 DOI: https://doi.org/10.1097/00002030-200109070-00008

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Abstract

OBJECTIVES: To quantify the percentage of the two major subpopulations of blood dendritic cells (DC) in HIV-1-seropositive Ugandan individuals infected with non-clade B viruses and compare this with that seen in clade B HIV-1 infected non-African individuals. DC maturation/activation status was also investigated via the expression of CD86. METHODS: The percentage of blood DC was quantified by using flow cytometry. DC were identified as the lineage (CD3, CD14, CD16, CD19, CD20, CD56)-negative, HLA-DR-positive population and the two major subpopulations were differentiated by CD11c expression. RESULTS: The percentage of blood DC was reduced significantly in HIV-1-seropositive African individuals when compared with controls (0.21 and 0.39% respectively). A similar reduction was also seen in non-African patients residing in the UK (0.19% compared with 0.36% for controls). However, there was no selective loss in either CD11c-positive or CD11c-negative subpopulations. The percentage of blood DC expressing CD86 was significantly greater in HIV-1-seropositive individuals when compared with controls and the increased expression was largely confined to CD11c-negative DC. CONCLUSIONS: Africans infected with non-clade B HIV-1 showed similar reductions in the percentage of blood DC to non-Africans infected with clade B viruses. There was no selective loss of either DC subpopulation, suggesting that the ability of DC to acquire and present antigens or to produce interferon-alpha may both be impaired in HIV-1 infection.

Item Type: Article
Keywords: Adult, Africa, Antigens, CD/metabolism, CD4 Lymphocyte Count, Cell Differentiation, Comparative Study, Dendritic Cells/cytology/*physiology, Female, Flow Cytometry, HIV Antibodies/blood, HIV Infections/*immunology/virology, *HIV-1/immunology, Human, Integrin alphaXbeta2/metabolism, Male, Membrane Glycoproteins/metabolism, RNA, Viral/blood, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S., Adult, Africa, Antigens, CD, metabolism, CD4 Lymphocyte Count, Cell Differentiation, Comparative Study, Dendritic Cells, cytology, physiology, Female, Flow Cytometry, HIV Antibodies, blood, HIV Infections, immunology, virology, HIV-1, immunology, Human, Integrin alphaXbeta2, metabolism, Male, Membrane Glycoproteins, metabolism, RNA, Viral, blood, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S.
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
PubMed ID: 11546940
Web of Science ID: 171472800008
URI: http://researchonline.lshtm.ac.uk/id/eprint/16055

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