Safety and immunogenicity of the candidate tuberculosis vaccine MVA85A in West Africa.


Brookes, RH; Hill, PC; Owiafe, PK; Ibanga, HB; Jeffries, DJ; Donkor, SA; Fletcher, HA; Hammond, AS; Lienhardt, C; Adegbola, RA; McShane, H; Hill, AV; (2008) Safety and immunogenicity of the candidate tuberculosis vaccine MVA85A in West Africa. PLoS One, 3 (8). e2921. ISSN 1932-6203 DOI: https://doi.org/10.1371/journal.pone.0002921

[img]
Preview
Text - Published Version
License:

Download (335kB) | Preview

Abstract

BACKGROUND Vaccination with a recombinant modified vaccinia Ankara expressing antigen 85A from Mycobacterium tuberculosis, MVA85A, induces high levels of cellular immune responses in UK volunteers. We assessed the safety and immunogenicity of this new vaccine in West African volunteers. METHODS AND FINDINGS We vaccinated 21 healthy adult male subjects (11 BCG scar negative and 10 BCG scar positive) with MVA85A after screening for evidence of prior exposure to mycobacteria. We monitored them over six months, observing for clinical, haematological and biochemical adverse events, together with assessment of the vaccine induced cellular immune response using ELISPOT and flow cytometry. MVA85A was well tolerated with no significant adverse events. Mild local and systemic adverse events were consistent with previous UK trials. Marked immunogenicity was found whether individuals had a previous BCG scar or not. There was not enhanced immunogenicity in those with a BCG scar, and induced T cell responses were better maintained in apparently BCG-naïve Gambians than previously studied BCG-naïve UK vaccinees. Although responses were predominantly attributable to CD4+ T cells, we also identified antigen specific CD8+ T cell responses, in subjects who were HLA B-35 and in whom enough blood was available for more detailed immunological analysis. CONCLUSIONS These data on the safety and immunogenicity of MVA85A in West Africa support its accelerated development as a promising booster vaccine for tuberculosis. TRIAL REGISTRATION ClinicalTrials.gov NCT00423839.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
PubMed ID: 18698342
Web of Science ID: 264412600007
URI: http://researchonline.lshtm.ac.uk/id/eprint/1217149

Statistics


Download activity - last 12 months
Downloads since deposit
191Downloads
298Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item