A live experimental vaccine against Burkholderia pseudomallei elicits CD4(+) T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins


Haque, A; Chu, K; Easton, A; Stevens, MP; Galyov, EE; Atkins, T; Titball, R; Bancroft, GJ; (2006) A live experimental vaccine against Burkholderia pseudomallei elicits CD4(+) T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins. The Journal of infectious diseases, 194 (9). pp. 1241-1248. ISSN 0022-1899 DOI: 10.1086/508217

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Abstract

Burkholderia pseudomallei is the etiological agent of melioidosis, a serious human disease for which no vaccine is available. Immunization of susceptible BALB/c mice with the live attenuated mutant B. pseudomallei ilvI (referred to as "2D2") generated significant, although incomplete, immunity. Splenic B. pseudomallei-specific T cells, detected in immunized mice, proliferated and produced interferon-gamma in vitro in response to dead bacteria. Assessment of T cell antigen specificity indicated that subpopulations of B. pseudomallei-reactive T cells were responsive to BopE, a type III secretion system (TTSS) effector protein, and to a lesser extent to BipD, a TTSS translocator protein. Increased survival of severe combined immunodeficient mice adoptively transferred with T cells from immunized mice, compared with that of naive T cell recipients, demonstrated that immunization with 2D2 generated T cell-mediated immunity. CD4(+) and CD8(+) cell depletion studies demonstrated that CD4(+) cells, but not CD8(+) cells, mediated this protection in vivo. Thus, CD4(+) T cells can mediate vaccine-induced immunity to experimental melioidosis.

Item Type: Article
Keywords: SALMONELLA-TYPHIMURIUM, CAPSULAR POLYSACCHARIDE, MURINE MODEL, BALB/C, MICE, INFECTION, MELIOIDOSIS, PROTECTION, RESPONSES, INVASION, LIPOPOLYSACCHARIDE, Animals, Bacterial Proteins, immunology, Bacterial Vaccines, immunology, Burkholderia Infections, immunology, prevention & control, Burkholderia pseudomallei, immunology, CD4-Positive T-Lymphocytes, immunology, metabolism, Female, Interferon Type II, metabolism, Mice, Mice, Inbred BALB C, Spleen, metabolism, microbiology, Virulence
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Vaccine Centre
PubMed ID: 17041850
Web of Science ID: 241418100009
URI: http://researchonline.lshtm.ac.uk/id/eprint/10507

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