Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions.
Antonio, Martin;
Hakeem, Ishrat;
Sankareh, Kawsu;
Cheung, Yin Bun;
Adegbola, Richard A;
(2009)
Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions.
Journal of medical microbiology, 58 (Pt 3).
pp. 296-302.
ISSN 0022-2615
DOI: https://doi.org/10.1099/jmm.0.006031-0
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Sequential multiplex PCR was evaluated for detection of multiple Streptococcus pneumoniae serotypes directly from nasopharyngeal secretions. A total of 279 nasopharyngeal swab samples were tested blindly. When limited to the 29 serotypes identifiable by the molecular method, the mean number of serotypes identified by the conventional latex/Quellung method was 0.85, which was significantly lower than that by the molecular method (P <0.0001). The multiplex PCR method identified significantly more serotypes than the latex/Quellung method if limited to the 29 serotypes (P=0.001 and P=0.014, respectively).
