Barton, Amber; Rosenkrands, Ida; Pickering, Harry; Faal, Nkoyo; Harte, Anna; Joof, Hassan; Makalo, Pateh; Ragonnet, Manon; Olsen, Anja Weinreich; Bailey, Robin L; +4 more... Mabey, David CW; Follmann, Frank; Dietrich, Jes; Holland, Martin J; (2023) A systems serology approach to the investigation of infection-induced antibody responses and protection in trachoma. Frontiers in immunology, 14. 1178741-. ISSN 1664-3224 DOI: https://doi.org/10.3389/fimmu.2023.1178741
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Abstract
BACKGROUND: Ocular infections with Chlamydia trachomatis serovars A-C cause the neglected tropical disease trachoma. As infection does not confer complete immunity, repeated infections are common, leading to long-term sequelae such as scarring and blindness. Here, we apply a systems serology approach to investigate whether systemic antibody features are associated with susceptibility to infection. METHODS: Sera from children in five trachoma endemic villages in the Gambia were assayed for 23 antibody features: IgG responses towards two C. trachomatis antigens and three serovars [elementary bodies and major outer membrane protein (MOMP), serovars A-C], IgG responses towards five MOMP peptides (serovars A-C), neutralization, and antibody-dependent phagocytosis. Participants were considered resistant if they subsequently developed infection only when over 70% of other children in the same compound were infected. RESULTS: The antibody features assayed were not associated with resistance to infection (false discovery rate < 0.05). Anti-MOMP SvA IgG and neutralization titer were higher in susceptible individuals (p < 0.05 before multiple testing adjustment). Classification using partial least squares performed only slightly better than chance in distinguishing between susceptible and resistant participants based on systemic antibody profile (specificity 71%, sensitivity 36%). CONCLUSIONS: Systemic infection-induced IgG and functional antibody responses do not appear to be protective against subsequent infection. Ocular responses, IgA, avidity, or cell-mediated responses may play a greater role in protective immunity than systemic IgG.
Item Type | Article |
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Faculty and Department | Faculty of Infectious and Tropical Diseases > Dept of Clinical Research |
Research Centre | Centre for Maternal, Reproductive and Child Health (MARCH) |
PubMed ID | 37287960 |
Elements ID | 204245 |
Official URL | http://dx.doi.org/10.3389/fimmu.2023.1178741 |
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