Septins are an evolutionarily conserved protein family whose members form hetero-oligomeric complexes that assemble into filaments and higher-order structures. In this issue, Martins et al. (2022. J. Cell Biol.https://doi.org/10.1083/jcb.202203016) and Cannon et al. (2023. J. Cell Biol.https://doi.org/10.1083/jcb.202204063) report that heteromer composition impacts the physiological role of septin filaments in yeast and human cells.