Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men.
Birtles, Andrew;
McCarthy, Noel;
Sheppard, Carmen L;
Rutter, Harry;
Guiver, Malcolm;
Haworth, Elizabeth;
George, Robert C;
(2005)
Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men.
Journal of clinical microbiology, 43 (4).
pp. 2004-2008.
ISSN 0095-1137
DOI: https://doi.org/10.1128/JCM.43.4.2004-2008.2005
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Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.