Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses.

Francis Kasolo ; Kennedy Lishimpi ; Chifumbe Chintu ; Peter Mwaba ; Victor Mudenda ; Daniel Maswahu ; Hiroshi Terunuma ; Helen Fletcher ORCID logo ; Andrew Nunn ; Sebastian Lucas ; +1 more... Alimuddin Zumla ; (2002) Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses. The Journal of pediatrics, 140 (3). pp. 367-369. ISSN 0022-3476 DOI: 10.1067/mpd.2002.122468
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Polymerase chain reaction for Pneumocystis carinii DNA was performed on necropsy lung samples from children by means of P carinii -specific primers.P carinii DNA was identified in 22 of 22 (100%) samples with histologically proven P carinii pneumonia and 13 of 75 (17%) with non-P carinii pneumonia respiratory illness (sensitivity, 100%; specificity, 83%). The low specificity precludes the use of polymerase chain reaction as an alternative to histopathologic diagnosis.
Item Type Article
ISI 175061500018
Date Deposited 03 Jul 2014 20:26

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