Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses.
Kasolo, F; Lishimpi, K; Chintu, C; Mwaba, P; Mudenda, V; Maswahu, D; Terunuma, H; Fletcher, H
; Nunn, A; Lucas, S; +1 more...Zumla, A and
(2002)
Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses.
The Journal of pediatrics, 140 (3).
pp. 367-369.
ISSN 0022-3476
DOI: 10.1067/mpd.2002.122468
Polymerase chain reaction for Pneumocystis carinii DNA was performed on necropsy lung samples from children by means of P carinii -specific primers.P carinii DNA was identified in 22 of 22 (100%) samples with histologically proven P carinii pneumonia and 13 of 75 (17%) with non-P carinii pneumonia respiratory illness (sensitivity, 100%; specificity, 83%). The low specificity precludes the use of polymerase chain reaction as an alternative to histopathologic diagnosis.
Item Type | Article |
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ISI | 175061500018 |
Date Deposited | 03 Jul 2014 20:26 |
ORCID: https://orcid.org/0000-0002-0435-1006