Brazil's first free-mating laboratory colony of Nyssorhynchus darlingi.
Araujo, Maisa da Silva;
Andrade, Alice Oliveira;
Santos, Najara Akira Costa Dos;
Pereira, Dhélio Batista;
Costa, Glaucilene da Silva;
Paulo, Paula Frassinetti Medeiros de;
Rios, Carlos Tong;
Moreno, Marta;
Pereira-da-Silva, Luiz Hidelbrando;
Medeiros, Jansen Fernandes de;
(2019)
Brazil's first free-mating laboratory colony of Nyssorhynchus darlingi.
REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 52.
e20190159-.
ISSN 0037-8682
DOI: https://doi.org/10.1590/0037-8682-0159-2019
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INTRODUCTION: The lack of highly-productive Nyssorhynchus darlingi laboratory colonies limits some studies. We report the first well-established laboratory colony of Ny. darlingi in Brazil. METHODS: Mosquitoes were collected from Porto Velho and were reared at the Laboratory of Fiocruz/RO. After induced mating by light stimulation in the F1 to F6, the subsequent generations were free mating. Larvae were reared in distilled water and fed daily until pupation. RESULTS: In 11 generations, the colony produced a high number of pupae after the F5 generation. CONCLUSIONS: These results demonstrate the potential for permanently establishing Ny. darlingi colonies for research purposes in Brazil.