Performance of the HerpeSelect (Focus) and Kalon Enzyme-Linked Immunosorbent Assays for Detection of Antibodies Against Herpes Simplex Virus Type 2 Using Monoclonal Antibody-Blocking Enzyme Immunoassay (MAb-EIA) and Clinico-Virological Reference Standards in Brazil.


Nascimento, MC; Ferreira, S; Sabino, E; Hamilton, I; Parry, J; Pannuti, CS; Mayaud, P; (2007) Performance of the HerpeSelect (Focus) and Kalon Enzyme-Linked Immunosorbent Assays for Detection of Antibodies Against Herpes Simplex Virus Type 2 Using Monoclonal Antibody-Blocking Enzyme Immunoassay (MAb-EIA) and Clinico-Virological Reference Standards in Brazil. Journal of clinical microbiology, 45 (7). pp. 2309-11. ISSN 0095-1137 DOI: https://doi.org/10.1128/JCM.00144-07

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Abstract

A total of 586 serum samples were used to evaluate the performance of type-specific herpes simplex virus type 2 (HSV-2) commercial enzyme-linked immunosorbent assays (ELISAs) by using the monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and a clinicovirological panel as reference standards. The Kalon and HerpeSelect ELISAs had similar sensitivities (93.5% and 93.8% compared with the results obtained by MAb-EIA, respectively, and 100% for both ELISAs compared with the results obtained with a clinicovirological panel). The Kalon ELISA had a higher specificity (96.5% and 96.8% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively) than the HerpeSelect ELISA (86.9% and 94% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively). A higher cutoff significantly improved the specificity of the HerpeSelect ELISA.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
Research Centre: Centre for Maternal, Reproductive and Child Health (MARCH)
PubMed ID: 17507516
Web of Science ID: 248072900041
URI: http://researchonline.lshtm.ac.uk/id/eprint/9925

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