Molecular approaches for a better understanding of the epidemiology and population genetics of Leishmania


Schonian, G; Kuhls, K; Mauricio, IL; (2011) Molecular approaches for a better understanding of the epidemiology and population genetics of Leishmania. Parasitology, 138 (4). pp. 405-425. ISSN 0031-1820 DOI: https://doi.org/10.1017/S0031182010001538

[img]
Preview
Text - Published Version
License:

Download (333kB) | Preview

Abstract

Molecular approaches are being used increasingly for epidemiological studies of visceral and cutaneous leishmaniases. Several molecular markers resolving genetic differences between Leishmania parasites at species and strain levels have been developed to address key epidemiological and population genetic questions. The current gold standard, multilocus enzyme typing (MLEE), needs cultured parasites and lacks discriminatory power. PCR assays identifying species directly with clinical samples have proven useful in numerous field studies. Multilocus sequence typing (MLST) is potentially the most powerful phylogenetic approach and will, most probably, replace MLEE in the future. Multilocus microsatellite typing (MLMT) is able to discriminate below the zymodeme level and seems to be the best candidate for becoming the gold standard for distinction of strains. Population genetic studies by MLMT revealed geographical and hierarchic population structure in L. tropica, L. major and the L. donovani complex. The existence of hybrids and gene flow between Leishmania populations suggests that sexual recombination is more frequent than previously thought. However, typing and analytical tools need to be further improved. Accessible databases should be created and sustained for integrating data obtained by different researchers. This would allow for global analyses and help to avoid biases in analyses due to small sample sizes.

Item Type: Article
Keywords: Leishmania, molecular markers, phylogeny, population genetics, multilocus sequence typing, multilocus microsatellite typing, POLYMERASE-CHAIN-REACTION, NEW-WORLD LEISHMANIA, INTERNAL TRANSCRIBED, SPACER, DONOVANI ZYMODEME MON-37, CUTANEOUS LEISHMANIASIS, VISCERAL, LEISHMANIASIS, MICROSATELLITE ANALYSIS, PCR ASSAY, CLINICAL-SAMPLES, SPECIES IDENTIFICATION
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Leishmaniasis Group
PubMed ID: 21078222
Web of Science ID: 288614600001
URI: http://researchonline.lshtm.ac.uk/id/eprint/986

Statistics


Download activity - last 12 months
Downloads since deposit
695Downloads
333Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item