Combined prime-boost vaccination against tick-borne encephalitis (TBE) using a recombinant vaccinia virus and a bacterial plasmid both expressing TBE virus non-structural NS1 protein


Aleshin, SE; Timofeev, AV; Khoretonenko, MV; Zakharova, LG; Pashvykina, GV; Stephenson, JR; Shneider, AM; Altstein, AD; (2005) Combined prime-boost vaccination against tick-borne encephalitis (TBE) using a recombinant vaccinia virus and a bacterial plasmid both expressing TBE virus non-structural NS1 protein. BMC Microbiol, 5. p. 45. ISSN 1471-2180 DOI: 10.1186/1471-2180-5-45

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Abstract

BACKGROUND: Heterologous prime-boost immunization protocols using different gene expression systems have proven to be successful tools in protecting against various diseases in experimental animal models. The main reason for using this approach is to exploit the ability of expression cassettes to prime or boost the immune system in different ways during vaccination procedures. The purpose of the project was to study the ability of recombinant vaccinia virus (VV) and bacterial plasmid, both carrying the NS1 gene from tick-borne encephalitis (TBE) virus under the control of different promoters, to protect mice against lethal challenge using a heterologous prime-boost vaccination protocol. RESULTS: The heterologous prime-boost vaccination protocol, using a VV recombinant and bacterial plasmid, both containing the NS1 TBE virus protein gene under the control of different promoters, achieved a high level of protection in mice against lethal challenge with a highly pathogenic TBE virus strain. No signs of pronounced TBE infection were detected in the surviving animals. CONCLUSION: Heterologous prime-boost vaccination protocols using recombinant VV and bacterial plasmids could be used for the development of flavivirus vaccines.

Item Type: Article
Keywords: Animals, Bacteria, Cytomegalovirus, Encephalitis Viruses, Tick-Borne, Encephalitis, Tick-Borne, Humans, Immunization, Secondary, Lethal Dose 50, Mice, Mice, Inbred BALB C, Plasmids, Vaccines, DNA, Vaccinia virus, Viral Nonstructural Proteins, Viral Vaccines, Animals, Bacteria, genetics, immunology, Cytomegalovirus, immunology, Encephalitis Viruses, Tick-Borne, immunology, Encephalitis, Tick-Borne, immunology, Humans, Immunization, Secondary, Lethal Dose 50, Mice, Mice, Inbred BALB C, Plasmids, Vaccines, DNA, toxicity, Vaccinia virus, immunology, Viral Nonstructural Proteins, immunology, toxicity, Viral Vaccines, toxicity
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Research Centre: Vaccine Centre
PubMed ID: 16076390
Web of Science ID: 231269100001
URI: http://researchonline.lshtm.ac.uk/id/eprint/8717

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