Seasonal Carriage of pfcrt and pfmdr1 Alleles in Gambian Plasmodium falciparum Imply Reduced Fitness of Chloroquine-Resistant Parasites.
Ord, R; Alexander, N; Dunyo, S; Hallett, R; Jawara, M; Targett, G; Drakeley, CJ; Sutherland, CJ; (2007) Seasonal Carriage of pfcrt and pfmdr1 Alleles in Gambian Plasmodium falciparum Imply Reduced Fitness of Chloroquine-Resistant Parasites. The Journal of infectious diseases, 196 (11). pp. 1613-1619. ISSN 0022-1899 DOI: 10.1086/522154
Background. Observations in natural Plasmodium falciparum populations after removal of failing drugs suggest that there is a fitness cost of drug resistance.Methods. To examine the effect of transient removal of drug pressure, we analyzed seasonal changes in the prevalence of chloroquine (CQ)-resistant parasite genotypes in The Gambia. Parasite isolates from 441 children presenting with uncomplicated falciparum malaria over 5 seasons (1998-2002) were linked to weekly rainfall data.Results. The prevalence of CQ-resistant parasites increased slightly over 5 years, with the 76T allele of pfcrt (odds ratio [OR] per year, 1.16; P=.03) and the 86Y allele of pfmdr1 (OR per year, 1.18; P=.02) becoming significantly more common. However, intraseasonal analysis showed that these alleles decreased in prevalence each dry season. Wild-type parasites with respect to both loci predominated as transmission began each year, with resistant parasites becoming more common as drug use increased. This pattern was seen for both pfcrt-76T (OR per week, 1.09; P=.001) and pfmdr1-86Y (OR per week, 1.07; P=.001) and could not be explained by seasonal changes in the clonal complexity of infections.Conclusions. The fitness cost of CQ resistance works against the persistence of resistant parasites through the dry season.
|Faculty and Department:||Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
|Research Centre:||Antimicrobial Resistance Centre (AMR)
Tropical Epidemiology Group
|Web of Science ID:||250965900007|
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