Cloning and sequencing alleles of the KIR2DL4 gene from genomic DNA samples


Roberts, CH; Madrigal, JA; Marsh, SG; (2007) Cloning and sequencing alleles of the KIR2DL4 gene from genomic DNA samples. Tissue antigens, 69 Suppl 1. pp. 88-91. ISSN 0001-2815 DOI: https://doi.org/10.1111/j.1399-0039.2006.762_2.x

Full text not available from this repository. (Request a copy)

Abstract

Killer-cell immunoglobulin-like receptor (KIR) genes are highly polymorphic and polymorphisms have been found in all KIR exons. Although less is known of the introns, these also appear to be polymorphic. To generate a comprehensive database of KIR genomic sequences, which will aid in the design of KIR typing reagents, we have established a method for cloning and sequencing of KIR genes from genomic DNA. We cloned and sequenced the entire KIR2DL4 gene from genomic DNA using long template touchdown PCR and high capacity cloning vectors. Overlapping secondary amplicons were modified to include a nucleotide analogue that reduced sequencing problems associated with secondary structure formation in the KIR sequence. Using a modified sequencing chemistry we were able to sequence approximately 11,000 bases confirming the previously published KIR2DL4*005 allele sequence.

Item Type: Article
Keywords: *Alleles, Cloning, Molecular, DNA/*genetics, DNA Primers, *Genome, Human, Humans, Killer Cells, Natural, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, Immunologic/*genetics, *Sequence Analysis, DNA, Alleles, Cloning, Molecular, DNA, genetics, DNA Primers, Genome, Human, Humans, Killer Cells, Natural, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, Immunologic, genetics, Receptors, KIR, Receptors, KIR2DL4, Sequence Analysis, DNA
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
PubMed ID: 17445175
Web of Science ID: 245754300027
URI: http://researchonline.lshtm.ac.uk/id/eprint/6739

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
276Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item