Disparate subcellular location of putative sortase substrates in Clostridium difficile.


Peltier, J; Shaw, HA; Wren, BW; Fairweather, NF; (2017) Disparate subcellular location of putative sortase substrates in Clostridium difficile. Sci Rep, 7 (1). p. 9204. ISSN 2045-2322 DOI: 10.1038/s41598-017-08322-1

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Abstract

Clostridium difficile is a gastrointestinal pathogen but how the bacterium colonises this niche is still little understood. Sortase enzymes covalently attach specific bacterial proteins to the peptidoglycan cell wall and are often involved in colonisation by pathogens. Here we show C. difficile proteins CD2537 and CD3392 are functional substrates of sortase SrtB. Through manipulation of the C-terminal regions of these proteins we show the SPKTG motif is essential for covalent attachment to the cell wall. Two additional putative substrates, CD0183 which contains an SPSTG motif, and CD2768 which contains an SPQTG motif, are not cleaved or anchored to the cell wall by sortase. Finally, using an in vivo asymmetric cleavage assay, we show that despite containing a conserved SPKTG motif, in the absence of SrtB these proteins are localised to disparate cellular compartments.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Antimicrobial Resistance Centre (AMR)
PubMed ID: 28835650
URI: http://researchonline.lshtm.ac.uk/id/eprint/4283480

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