Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.
Heal, William P;
Wickramasinghe, Sasala R;
Bowyer, Paul W;
Holder, Anthony A;
Smith, Deborah F;
Leatherbarrow, Robin J;
Tate, Edward W;
(2008)
Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.
Chemical communications (Cambridge, England) (4).
pp. 480-482.
ISSN 1359-7345
DOI: https://doi.org/10.1039/b716115h
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N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.