Burkholderia pseudomallei kynB plays a role in AQ production, biofilm formation, bacterial swarming and persistence.


Butt, A; Halliday, N; Williams, P; Atkins, HS; Bancroft, GJ; Titball, RW; (2015) Burkholderia pseudomallei kynB plays a role in AQ production, biofilm formation, bacterial swarming and persistence. Research in microbiology. ISSN 0923-2508 DOI: 10.1016/j.resmic.2015.11.002

Full text not available from this repository.

Abstract

Kynurenine formamidase (KynB) forms part of the kynurenine pathway which metabolises tryptophan to anthranilate. This metabolite can be used for downstream production of 2-alkyl-4-quinolone (AQ) signalling molecules that control virulence in Pseudomonas aeruginosa. Here we investigate the role of kynB in the production of AQs and virulence-associated phenotypes of Burkholderia pseudomallei K96243, the causative agent of melioidosis. Deletion of kynB resulted in reduced AQ production, increased biofilm formation, decreased swarming and increased tolerance to ciprofloxacin. Addition of exogenous anthranilic acid restored the biofilm phenotype, but not the persister phenotype. This study suggests the kynurenine pathway is a critical source of anthranilate and signalling molecules that may regulate B. pseudomallei virulence.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 26654915
Web of Science ID: 372890100002
URI: http://researchonline.lshtm.ac.uk/id/eprint/2478739

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
113Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item