Laser-assisted microdissection for the study of the ecology of parasites in their hosts.


Post, R J; Crainey, J L; Bivand, A; Renz, A; (2009) Laser-assisted microdissection for the study of the ecology of parasites in their hosts. Molecular ecology resources, 9 (2). pp. 480-6. ISSN 1755-098X

Full text not available from this repository.

Abstract

The population biology of internal parasites is difficult to study because the adult parasites are often inaccessible, deep within the host's body. Developing stages, such as eggs in the faeces or larvae in the skin are more easily obtained, but are difficult to handle because they are often very small and with a tough cuticle. This has limited their use in molecular ecology for estimating population biology parameters of the adults (their parents). We have used Onchocerca ochengi (a filarial nematode parasite of cattle) to describe a novel and generally applicable method of easily and conveniently isolating individual larvae (microfilariae) from the host using laser-assisted microdissection. Furthermore, we have been able to improve the isolation of DNA by using the laser to bisect the larva to release DNA from the tissues enclosed within the parasite cuticle, and in this way we have achieved amplification of fragments over 1400 bp, and routinely PCR-amplified single-copy sequences from 5% of the DNA from a single larva (the equivalent of approximately 15 nuclei), and regularly from 0.5%.

Item Type: Article
PubMed ID: 21564677
URI: http://researchonline.lshtm.ac.uk/id/eprint/20245

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
249Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item