Infectious prion protein in the filtrate even after 15 nm filtration.


Yunoki, M; Tanaka, H; Urayama, T; Kanai, Y; Nishida, A; Yoshikawa, M; Ohkubo, Y; Kawabata, Y; Hagiwara, K; Ikuta, K; (2010) Infectious prion protein in the filtrate even after 15 nm filtration. Biologicals, 38 (2). pp. 311-3. ISSN 1045-1056 DOI: https://doi.org/10.1016/j.biologicals.2009.10.018

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Abstract

The evaluation of the removal efficacy during manufacturing is important for the risk assessment of plasma products with respect to possible contamination by infectious prions, as recently reported in several papers on the potential for prion transmission through plasma products. Here, we evaluated a virus removal filter which has 15 nm pores. An antithrombin sample immediately prior to nano-filtration was spiked with prion material prepared in two different ways. The removal (log reduction factor) of prion infectivity using animal bioassays was >or=4.72 and 4.00 in two independent filtrations. However, infectivity was detected in both the pellet and supernatant following ultracentrifugation of the 15 nm filtered samples, indicating difficulty in complete removal. The data supports the conclusion that a certain amount of infectious prion protein is present as a smaller and/or soluble form (less than approximately 15 nm in diameter).

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 19931469
Web of Science ID: 277800400021
URI: http://researchonline.lshtm.ac.uk/id/eprint/2024

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