Enhanced contact hypersensitivity in human monocyte chemoattractant protein-1 transgenic mouse
Mizumoto, N; Iwabichi, K; Nakamura, H; Ato, M; Shibaki, A; Kawashima, T; Kobayashi, H; Iwabuchi, C; Ohkawara, A; Onoe, K; (2001) Enhanced contact hypersensitivity in human monocyte chemoattractant protein-1 transgenic mouse. Immunobiology, 204 (4). pp. 477-93. ISSN 0171-2985 DOI: 10.1078/0171-2985-00057Full text not available from this repository.
Monocyte chemoattractant protein (MCP)-1 is a chemotactic cytokine for monocytes, memoryT cells and dendritic cells (DC). However, the precise role of MCP-1 in a variety of immunological responses remains unclear. In the present study, we analyzed contact hypersensitivity (CHS) using human MCP-1 transgenic mice (hMCP-1Tgm) that constitutively produce high levels of hMCP-1 in the sera. Following 2,4-dinitrofluorobenzene (DNFB) sensitization, enhancement of CHS was demonstrated in Tgm as compared with that in non-Tgm. Anti-hMCP-1 antibodies significantly inhibited the CHS in Tgm. A prominent accumulation of B7-1+I-Ad+ Langerhans' cells (LC) bearing haptens was detected in draining lymph nodes (DLN) of Tgm 24 h after DNFB or fluorescein isothiocyanate (FITC) sensitization. Similar results were obtained with BALB/c mice administrated recombinant (r) hMCP-1. Langerhans' cells (LC) in the epidermal sheets of Tgm increased in size and expressed high levels of I-Ad and B7-1 12 h after FITC application compared with those of non-Tgm. After 18 h, the number of LC in the epidermis was reduced in Tgm. It was also shown that the B7-1 expression on LC of BALB/c mice was augmented after culture with rhMCP-1. These findings demonstrate that MCP-1 not only accelerates LC migration from epidermis into the DLN after sensitization with haptens but also up-regulates the I-Ad and B7-1 expressions, which results in the enhanced T cell activation and CHS.
|Keywords:||Animal, Antigens, CD80, immunology, Cell Division, Cell Movement, Cells, Cultured, Dendritic Cells, immunology, Dermatitis, Contact, immunology, Dinitrofluorobenzene, immunology, pharmacology, Haptens, immunology, Histocompatibility Antigens Class II, immunology, Human, Keratinocytes, cytology, immunology, Kinetics, Lymph Nodes, immunology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Monocyte Chemoattractant Protein-1, genetics, immunology, Monocytes, immunology, Support, Non-U.S. Gov't, T-Lymphocytes, cytology, Up-Regulation|
|Faculty and Department:||Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection|
|Web of Science ID:||172856200006|
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