Vaccination against type F botulinum toxin using attenuated Salmonella enterica var Typhimurium strains expressing the BoNT/F HC fragment


Foynes, S; Holley, JL; Garmory, HS; Titball, RW; Fairweather, NF; (2003) Vaccination against type F botulinum toxin using attenuated Salmonella enterica var Typhimurium strains expressing the BoNT/F HC fragment. Vaccine, 21 (11-12). pp. 1052-1059. ISSN 0264-410X DOI: 10.1016/S0264-410X(02)00622-9

Full text not available from this repository.

Abstract

The utility of the htrA, pagC and nirB promoters to direct the expression of the carboxy-terminal (H-C) fragment of botulinum toxin F (FHC) in Salmonella enterica var Typhimurium has been evaluated. Only low levels of serum antibody were induced after immunisation, and some protection against botulinum toxin type F was demonstrated after oral immunisation of mice with two doses of any of these recombinant Salmonella. Immunisation with two doses of recombinant Salmonella expressing FHC from the htrA promoter gave the greatest protection, against up to 10,000 mouse lethal doses of botulinum toxin type E These results demonstrate the feasibility of an orally delivered vaccine against botulinum toxin type E (C) 2002 Published by Elsevier Science Ltd.

Item Type: Article
Keywords: botulinum toxin, Salmonella, oral vaccine, High-level expression, neurotoxin type-a, tetanus toxin, pichia-pastoris, escherichia-coli, oral vaccination, promoters, binding, purification, candidate
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre: Vaccine Centre
PubMed ID: 12559779
Web of Science ID: 181058800004
URI: http://researchonline.lshtm.ac.uk/id/eprint/17738

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
313Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item