Discrimination of human pathogenic subspecies of Francisella tularensis by using restriction fragment length polymorphism


Thomas, R; Johansson, A; Neeson, B; Isherwood, K; Sjostedt, A; Ellis, J; Titball, RW; (2003) Discrimination of human pathogenic subspecies of Francisella tularensis by using restriction fragment length polymorphism. Journal of clinical microbiology, 41 (1). pp. 50-57. ISSN 0095-1137 DOI: 10.1128/jcm.41.1.50-57.2003

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Abstract

We describe the use of two insertion sequence elements (ISFtu1 and ISFtu2) in Francisella tularensis to type strains by restriction fragment length polymorphism (RFLP). The RFLP profiles of 17 epidemiologically unrelated isolates were determined and compared. Our results showed that RFLP profiles can be used to assign F. tularensis strains into five main groups corresponding to strains of F. tularensis subsp. tularensis, F. tularensis strain ATCC 6223, strains of F. tularensis subsp. holarctica, strains of F. tularensis subsp. holarctica from Japan, and F. tularensis subsp. mediaasiatica. The results confirm the genetic identities of these subspecies and also support the suggestion that strains of F. tularensis subsp. holarctica from Japan should be considered members of a separate biovar. These findings should support future studies to determine the genetic differences between strains of F. tularensis at the whole-genome level.

Item Type: Article
Keywords: Insertion sequences, tularemia, strains, mycobacterium, japan, epidemiology, repeats, ticks, pcr
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 12517824
Web of Science ID: 180406700007
URI: http://researchonline.lshtm.ac.uk/id/eprint/17737

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