Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.
Fanello, C;
Santolamazza, F;
della Torre, A;
(2002)
Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.
Medical and veterinary entomology, 16 (4).
pp. 461-464.
ISSN 0269-283X
DOI: https://doi.org/10.1046/j.1365-2915.2002.00393.x
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For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.