Immune responses and protection induced by mucosal and systemic immunisation with recombinant measles nucleoprotein in a mouse model of measles virus-induced encephalitis


Olszewska, W; Erume, J; Ripley, J; Steward, MW; Partidos, CD; (2001) Immune responses and protection induced by mucosal and systemic immunisation with recombinant measles nucleoprotein in a mouse model of measles virus-induced encephalitis. Archives of virology, 146 (2). pp. 293-302. ISSN 0304-8608 DOI: https://doi.org/10.1007/s007050170176

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Abstract

In this study the immunogenicity of recombinant nucleoprotein (Np) administered intranasally or intraperitoneally, and its ability to support a systemic protective anti-virus antibody response was examined, in a mouse model of measles virus (MV)-induced encephalitis. Although both intranasal and intraperitoneal routes of immunisation resulted in priming Np- and MV-specific T-cell responses, the intraperitoneal route was shown to prime for a predominantly IgG2a serum anti-MV antibody response of high avidity, which confered complete protection following intracranial challenge with a neuroadapted strain of MV. On the other hand, intranasal priming resulted in a mixed IgG1, IgG2a serum anti-MV antibody response of low avidity, and only 43% of immunised mice survived following intracranial challenge with the neuroadapted strain of MV. These findings suggest that the route of immunisation in combination with an appropriate adjuvant could influence the induction of a quality antibody response with protective capacity.

Item Type: Article
Keywords: Animal, Antibodies, Viral/blood, Antibody Affinity, Antibody Formation, Brain/virology, Cercopithecus aethiops, Disease Models, Animal, Encephalitis, Viral/blood/*immunology, Immunity, Mucosal, Immunoglobulin G/blood/classification, Measles/blood/*immunology, Measles virus/growth & development/immunology, Mice, Nucleoproteins/*immunology, Recombinant Proteins/immunology, T-Lymphocytes/*immunology, Viral Proteins/*immunology, Animal, Antibodies, Viral, blood, Antibody Affinity, Antibody Formation, Brain, virology, Cercopithecus aethiops, Disease Models, Animal, Encephalitis, Viral, blood, immunology, Immunity, Mucosal, Immunoglobulin G, blood, classification, Measles, blood, immunology, Measles virus, growth & development, immunology, Mice, Nucleoproteins, immunology, Recombinant Proteins, immunology, T-Lymphocytes, immunology, Viral Proteins, immunology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
PubMed ID: 11315639
Web of Science ID: 167411800008
URI: http://researchonline.lshtm.ac.uk/id/eprint/17574

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