Entamoeba moshkovskii infections in children, Bangladesh


Ali, IK; Hossain, MB; Roy, S; Ayeh-Kumi, PF; Petri, W. A., Jr; Haque, R; Clark, CG; (2003) Entamoeba moshkovskii infections in children, Bangladesh. Emerging infectious diseases, 9 (5). pp. 580-4. ISSN 1080-6040

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Abstract

Entamoeba moshkovskii cysts are morphologically indistinguishable from those of the disease-causing species E. histolytica and the nonpathogenic E. dispar. Although sporadic cases of human infection with E. moshkovskii have been reported, the organism is considered primarily a free-living amoeba. No simple molecular detection tool is available for diagnosing E. moshkovskii infections. We used polymerase chain reaction (PCR) to detect E. moshkovskii directly in stool. We tested 109 stool specimens from preschool children in Bangladesh by PCR; 17 were positive for E. histolytica (15.6%) and 39 were positive for E. dispar (35.8%). In addition, we found that 23 (21.1%) were positive for E. moshkovskii infection, and 17 (73.9%) of these also carried E. histolytica or E. dispar. The high association of E. moshkovskii with E. histolytica and E. dispar may have obscured its identification in previous studies. The high prevalence found in this study suggests that humans may be a true host for this amoeba.

Item Type: Article
Keywords: Animal, Bangladesh/epidemiology, Base Sequence, Child, Preschool, DNA, Protozoan/analysis/genetics, Entamoeba/classification/enzymology/genetics/*isolation & purification, Entamoebiasis/*diagnosis/epidemiology/*parasitology, Feces/parasitology, Female, Human, Infant, Isoenzymes/analysis, Male, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Alignment, Species Specificity, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S., Animal, Bangladesh, epidemiology, Base Sequence, Child, Preschool, DNA, Protozoan, analysis, genetics, Entamoeba, classification, enzymology, genetics, isolation & purification, Entamoebiasis, diagnosis, epidemiology, parasitology, Feces, parasitology, Female, Human, Infant, Isoenzymes, analysis, Male, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Alignment, Species Specificity, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S.
Faculty and Department: Faculty of Infectious and Tropical Diseases
Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 12737742
Web of Science ID: 182569700011
URI: http://researchonline.lshtm.ac.uk/id/eprint/17191

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