Characterisation of a human herpesvirus 6 variant A 'amplicon' and replication modulation by U94-Rep 'latency gene'


Turner, S; Diluca, D; Gompels, U; (2002) Characterisation of a human herpesvirus 6 variant A 'amplicon' and replication modulation by U94-Rep 'latency gene'. Journal of virological methods, 105 (2). pp. 331-41. ISSN 0166-0934 DOI: https://doi.org/10.1016/S0166-0934(02)00130-1

Full text not available from this repository. (Request a copy)

Abstract

The human herpesvirus 6 (HHV-6) variant A genome has conserved sequences which are signals for initiating lytic replication (origin, 'ori-lyt') and DNA packaging into the virion (pac2/1). Here these are functionally characterised and used to construct a gene-expression amplifiable-vector, an 'amplicon', with applications for gene delivery to lymphoid-myeloid cells or their progenitor stem cells. A minimal efficient ori-lyt for replication was identified which was enhanced in the presence of the imperfect direct repeated DNA domain (IDR). In A variant strains these are arranged as three adjacent repeats with the most divergence in IDR3. Addition of the pac2/1 sequences also enhanced detection of ori-lyt replication and conferred DNA packaging properties, thus, the amplicon could be packaged with 'helper' virus. An HHV-6 specific factor, which inhibits amplicon replication was identified by trans replication assays. This is the U94-Rep 'latency' gene product, which can modulate efficiency of such amplifiable vectors, based on the lytic origin. It could also affect maintenance of viral genomes or vectors during latency.

Item Type: Article
Keywords: Base Sequence, DNA Primers, Gene Amplification, Herpesvirus 6, Human/*classification/*genetics/isolation & purification, Human, Jurkat Cells, Plasmids, Polymerase Chain Reaction/methods, Restriction Mapping/methods, Support, Non-U.S. Gov't, T-Lymphocytes/virology, *Variation (Genetics), Virus Replication/*physiology, Base Sequence, DNA Primers, Gene Amplification, Herpesvirus 6, Human, classification, genetics, isolation & purification, Human, Jurkat Cells, Plasmids, Polymerase Chain Reaction, methods, Restriction Mapping, methods, Support, Non-U.S. Gov't, T-Lymphocytes, virology, Variation (Genetics), Virus Replication, physiology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 12270665
Web of Science ID: 178438700014
URI: http://researchonline.lshtm.ac.uk/id/eprint/17020

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
305Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item