A role for human cytomegalovirus glycoprotein O (gO) in cell fusion and a new hypervariable locus


Paterson, DA; Dyer, AP; Milne, RS; Sevilla-Reyes, E; Gompels, UA; (2002) A role for human cytomegalovirus glycoprotein O (gO) in cell fusion and a new hypervariable locus. Virology, 293 (2). pp. 281-94. ISSN 0042-6822 DOI: https://doi.org/10.1006/viro.2001.1274

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Abstract

A cell fusion assay using fusion-from-without (FFWO) recombinant adenoviruses (RAds) and specific antibody showed a role in fusion modulation for glycoprotein gO, the recently identified third component of the gH/gL gCIII complex of human cytomegalovirus (HCMV). As in HCMV, RAd gO expressed multiple glycosylated species with a mature product of 125 kDa. Coexpression with gH/gL RAds showed gCIII reconstitution in the absence of other HCMV products and stabilisation by intermolecular disulfide bonds. Properties of HCMV clinical isolate, Pt, also implicated gO in cell spread. Compared to laboratory strain AD169, Pt was resistant to gH antibody plaque inhibition, but mature gH was identical. However, the gO sequences were highly divergent (20%), with further variation in laboratory strain Towne gO (34%). Thus, gO forms gCIII with gH/gL, performs in cell fusion, and is a newly identified HCMV hypervariable locus which may influence gCIII's function in mediating infection.

Item Type: Article
Keywords: Adenoviridae/genetics, Amino Acid Sequence, *Cell Fusion, Cell Line, Comparative Study, Complementarity Determining Regions, Cytomegalovirus/chemistry/genetics/*physiology, Genes, Viral, Human, Membrane Glycoproteins/chemistry/genetics/*physiology, Molecular Sequence Data, Recombination, Genetic, Sequence Alignment, Support, Non-U.S. Gov't, Variation (Genetics), Viral Envelope Proteins/chemistry/genetics/*physiology, Adenoviridae, genetics, Amino Acid Sequence, Cell Fusion, Cell Line, Comparative Study, Complementarity Determining Regions, Cytomegalovirus, chemistry, genetics, physiology, Genes, Viral, Human, Membrane Glycoproteins, chemistry, genetics, physiology, Molecular Sequence Data, Recombination, Genetic, Sequence Alignment, Support, Non-U.S. Gov't, Variation (Genetics), Viral Envelope Proteins, chemistry, genetics, physiology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 11886248
Web of Science ID: 174378100010
URI: http://researchonline.lshtm.ac.uk/id/eprint/17019

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