Decreased IFN-gamma and increased IL-4 production by human CD8(+) T cells in response to Mycobacterium tuberculosis in tuberculosis patients


Smith, SM; Klein, MR; Malin, AS; Sillah, J; McAdam, K; Dockrell, HM; (2002) Decreased IFN-gamma and increased IL-4 production by human CD8(+) T cells in response to Mycobacterium tuberculosis in tuberculosis patients. Tuberculosis (Edinburgh, Scotland), 82 (1). pp. 7-13. ISSN 1472-9792 DOI: https://doi.org/10.1054/tube.2001.0317

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Abstract

To investigate the role of MHC class I restricted CD8(+) T cells in host defense to M. tuberculosis, peripheral blood mononuclear cells (PBMC) from healthy BCG-vaccinated donors and untreated pulmonary tuberculosis (TB) patients in The Gambia were stimulated for 6 days with M. bovis BCG or M. tuberculosis and the CD8(+) T cell response analyzed. Intracellular FACS analysis of cytokine production by CD8(+) T cells showed that IFN-gamma and TNF-alpha production were greatly reduced in TB patients compared to healthy controls. IL-4-producing CD8(+) T cells were detected in TB patients, a phenotype absent in controls. Collectively, these data suggest that an alteration in the type 1/ type 2 cytokine balance occurs in CD8(+) T cells during clinical tuberculosis, and that this may provide a surrogate marker for disease. (C) 2002, Elsevier Science Ltd. All rights reserved.

Item Type: Article
Keywords: Blood mononuclear-cells, cytokine responses, immune-response, pulmonary tuberculosis, schistosoma-mansoni, human infection, in-vitro, resistance, antigens, mice, Adult, CD8-Positive T-Lymphocytes, immunology, Flow Cytometry, Human, Immunity, Cellular, Interferon Type II, biosynthesis, Interleukin-4, biosynthesis, Male, Mycobacterium tuberculosis, immunology, Support, Non-U.S. Gov't, Tuberculosis, immunology, microbiology, Tumor Necrosis Factor, biosynthesis
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
Academic Services & Administration > Academic Administration
Research Centre: TB Centre
PubMed ID: 11914057
Web of Science ID: 175235900002
URI: http://researchonline.lshtm.ac.uk/id/eprint/16436

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