Differentiation of Entamoeba histolytica/Entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis
Sanchez-Guillen, MD; Perez-Fuentes, R; Salgado-Rosas, H; Ruiz-Arguelles, A; Ackers, JP; Shire, AM; Talamas-Rohana, P; (2002) Differentiation of Entamoeba histolytica/Entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis. The American journal of tropical medicine and hygiene, 66 (6). pp. 731-7. ISSN 0002-9637Full text not available from this repository.
To correlate a particular state of immunity with Entamoeba spp., we used colorimetric PCR to differentiate E. histolytica from E. dispar in individuals with amoebiasis and to associate its presence with the clinical profile, including humoral and cellular immune responses to E. histolytica. Our results showed high levels of antibody in acute amoebiasis and elevation of IL-4 production, a cytokine related to Th2 profile, associated with E. histolytica. In chronic amoebiasis, even with anti-E. histolytica seropositivity, intestinal symptoms were associated with E. dispar in all the cases, without differences in level of antibodies, BTI, CD4+/CD8+ ratio, INF-gamma, and IL-4. Among asymptomatic carriers, E. dispar was more frequently found; however, identification of E. histolytica in two asymptomatic carriers associated with high levels of INF-gamma, a cytokine related to Th1 profile, demonstrate the importance of making specific diagnosis of Entamoeba spp., to establish the clinical and epidemiological behavior in both intestinal and extra-intestinal amoebiasis.
|Keywords:||Polymerase chain-reaction, amebic liver-abscess, intestinal, amebiasis, interleukin-2 production, interferon-gamma, peripheral-blood, lymphocyte-t, in-vivo, infection, diagnosis, Acute Disease, Animal, Antibodies, Protozoan, blood, Antibody Formation, CD4-CD8 Ratio, Chronic Disease, Comparative Study, Cytokines, blood, Dysentery, Amebic, immunology, physiopathology, Entamoeba, classification, genetics, immunology, Entamoeba histolytica, classification, genetics, immunology, Entamoebiasis, diagnosis, immunology, Enzyme-Linked Immunosorbent Assay, Human, Immunity, Cellular, Interferon Type II, blood, Interleukin-4, blood, Mexico, Polymerase Chain Reaction, methods, Support, Non-U.S. Gov't, T-Lymphocytes, immunology, Time Factors|
|Faculty and Department:||Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology|
|Web of Science ID:||177660700017|
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