Salmonella enterica serovar typhimurium expressing a chromosomally integrated copy of the Bacillus anthracis protective antigen gene protects mice against an anthrax spore challenge


Garmory, HS; Titball, RW; Griffin, KF; Hahn, U; Bohm, R; Beyer, W; (2003) Salmonella enterica serovar typhimurium expressing a chromosomally integrated copy of the Bacillus anthracis protective antigen gene protects mice against an anthrax spore challenge. Infection and immunity, 71 (7). pp. 3831-3836. ISSN 0019-9567 DOI: 10.1128/iai.71.7.3831-3836.2003

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Abstract

Protective immunity against infection with Bacillus anthracis is almost entirely based on a response to the protective antigen (PA), the binding moiety for the two other toxin components. We cloned the PA gene into an auxotrophic mutant of Salmonella enterica serovar Typhimurium as a fusion with the signal sequence of the hemolysin (Hly) A gene of Escherichia coli to allow the export of PA via the Hly export system. To stabilize the export cassette, it was also integrated into the chromosome of the live Salmonella carrier. When S. enterica serovar Typhimurium with the chromosomally integrated PA gene was given intravenously to A/J mice, they developed high levels of antibody to PA. These mice were protected against intraperitoneal challenge with 100 or 1,000 50% lethal doses of B. anthracis strain STI. This work contributes to the development of a Salmonella-based orally delivered anthrax vaccine.

Item Type: Article
Keywords: antibody-responses, fusion protein, secretion, infection, efficacy, vaccines, immunization, immunity, sequence, subunit, Animal, Anthrax, prevention & control, Anthrax Vaccines, immunology, Antigens, Bacterial, genetics, immunology, Bacillus anthracis, genetics, immunology, Bacterial Toxins, genetics, immunology, Female, Mice, Salmonella typhimurium, genetics, Spores, Bacterial, Support, Non-U.S. Gov't, Vaccines, Synthetic, immunology
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 12819066
Web of Science ID: 183797200020
URI: http://researchonline.lshtm.ac.uk/id/eprint/16187

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