Characterization of the O antigen gene cluster and structural analysis of the O antigen of Francisella tularensis subsp. tularensis


Prior, JL; Prior, RG; Hitchen, PG; Diaper, H; Griffin, KF; Morris, HR; Dell, A; Titball, RW; (2003) Characterization of the O antigen gene cluster and structural analysis of the O antigen of Francisella tularensis subsp. tularensis. Journal of medical microbiology, 52 (Pt 10). pp. 845-51. ISSN 0022-2615 DOI: 10.1099/jmm.0.05184-0

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Abstract

A gene cluster encoding enzymes involved in LPS O antigen biosynthesis was identified from the partial genome sequence of Francisella tularensis subsp. tularensis Schu S4. All of the genes within the cluster were assigned putative functions based on sequence similarity with genes from O antigen biosynthetic clusters from other bacteria. Ten pairs of overlapping primers were designed to amplify the O antigen biosynthetic cluster by PCR from nine strains of F. tularensis. Although the gene cluster was present in all strains, there was a size difference in one of the PCR products between subsp. tularensis strains and subsp. holarctica strains. LPS was purified from F. tularensis subsp. tularensis Schu S4 and the O antigen was shown by mass spectrometry to have a structure similar to that of F. tularensis subsp. holarctica strain 15. When LPS from F. tularensis subsp. tularensis Schu S4 was used to immunize mice that were then challenged with F. tularensis subsp. tularensis Schu S4, an extended time to death was observed.

Item Type: Article
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 12972577
Web of Science ID: 186073900001
URI: http://researchonline.lshtm.ac.uk/id/eprint/16039

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