Genetic vaccination of mice with plasmids encoding the NS1 non- structural protein from tick-borne encephalitis virus and dengue 2 virus


Timofeev, AV; Butenko, VM; Stephenson, JR; (2004) Genetic vaccination of mice with plasmids encoding the NS1 non- structural protein from tick-borne encephalitis virus and dengue 2 virus. Virus genes, 28 (1). pp. 85-97. ISSN 0920-8569 DOI: https://doi.org/10.1023/B:VIRU.0000012266.04871.ce

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Abstract

Although there is a safe, inexpensive and efficacious vaccine against yellow fever, vaccination against other flavivirus diseases is less successful. There is no licensed vaccine against dengue fever and current vaccines against tick-borne encephalitis (TBE) and Japanese encephalitis are expensive and require several injections. Furthermore novel vaccines containing only virus envelope proteins may raise fears over antibody mediated enhancement (ADE) of disease. Here we report the successful use of genetic vaccination against TBE in an experimental animal model using a plasmid containing the coding sequence of a nonstructural protein (NS1). Such vaccines would provide inexpensive protection against disease, without raising concerns over inducing ADE on subsequent exposure to heterotypic infectious virus. Attempts to generate chaemeric plasmids to protect against both TBE and dengue fever were less successful. Although these chaemeric plasmids directed the synthesis and secretion of the virus NS1 protein normally, no protection was observed against either TBE or dengue fever.

Item Type: Article
Keywords: flavivirus, naked DNA, non-structural virus proteins, tick-, borneencephalitis, vaccine, cytotoxic t-lymphocytes, memory b-cells, japanese encephalitis, dna vaccine, protective immunity, envelope genes, viral, challenge, yellow-fever, neutralizing antibodies, monoclonal-, antibodies
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
PubMed ID: 14739654
Web of Science ID: 188213600007
URI: http://researchonline.lshtm.ac.uk/id/eprint/14997

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