Linkage of human cytomegalovirus glycoprotein gO variant groups identified from worldwide clinical isolates with gN genotypes, implications for disease associations and evidence for N- terminal sites of positive selection


Mattick, C; Dewin, D; Polley, S; Sevilla-Reyes, E; Pignatelli, S; Rawlinson, W; Wilkinson, G; Dal Monte, P; Gompels, UA; (2004) Linkage of human cytomegalovirus glycoprotein gO variant groups identified from worldwide clinical isolates with gN genotypes, implications for disease associations and evidence for N- terminal sites of positive selection. Virology, 318 (2). pp. 582-597. ISSN 0042-6822 DOI: https://doi.org/10.1016/j.virol.2003.09.036

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Abstract

Previously, we identified the glycoprotein gO gene, UL74, as a hypervariable locus in the human cytomegalovirus (HCMV) genome [Virology 293 (2002) 28 1]. Here, we analyze gO from 50 isolates from congenitally infected newborns, transplant recipients, and HIV/AIDS patients from Italy, Australia, and UK. These are compared to four gO groups described from USA transplantation patients [J. Virol. 76 (2002) 10841]. Phylogenetic analyses identified seven genotypes. Divergence between genotypes was up to 55% and within 3%. Discrete linkage was shown between seven hypervariable gO and gN genotypes, but not with gB. This suggests interactions, while gN and gO are known to form complexes with distinct conserved glycoproteins gM, gH/gL, respectively, both are involved in fusogenic entry and exit. Codon-based maximum likelihood models showed evidence for sites of positive selection. Further analyses of disease relationships should take into account these newly defined gO/gN groups. (C) 2003 Elsevier Inc. All rights reserved.

Item Type: Article
Keywords: cytomegalovirus, glycoprotein, strain variation, hypervariable, locus, gCIII complex, gCII complex, positive selection, virus, infection, CMV genotype, vaccine, Multiple sequence alignment, epstein-barr-virus, amino-acid, sites, endothelial-cells, in-vitro, pseudorabies virus, protein, sequences, genomic variants, 3rd component, gciii complex
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 14972526
Web of Science ID: 189164200012
URI: http://researchonline.lshtm.ac.uk/id/eprint/14875

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