Mortola, E; Oliva, G; Risso, M; Pecoraro, M; Venturini, MC; (2004) Feline immunodeficiency virus infection: a comparative study of different diagnostic techniques. Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 56 (1). 13-. https://researchonline.lshtm.ac.uk/id/eprint/14666
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Abstract
This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
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