Performance of commercially available enzyme Immunoassays for detection of antibodies against herpes simplex virus type 2 in African populations


van Dyck, E; Buve, A; Weiss, HA; Glynn, JR; Brown, DWG; de Deken, W; Parry, J; Hayes, RJ; (2004) Performance of commercially available enzyme Immunoassays for detection of antibodies against herpes simplex virus type 2 in African populations. Journal of clinical microbiology, 42 (7). p. 2961. ISSN 0095-1137 DOI: https://doi.org/10.1128/jcm.42.7.2961-2965.2004

Full text not available from this repository. (Request a copy)

Abstract

Data are accumulating on the performance of enzyme immunoassays (EIAs) for the detection of herpes simplex virus type 2 (HSV-2) infection in North America and Europe, but little is known about their performance in other populations. Nine test kits were evaluated with 330 serum samples from sub-Saharan Africa. The tests were first compared to the monoclonal antibody (MAII) EIA (Central Public Health Laboratory, London, United Kingdom). Samples that gave discordant results in the MAb EIA and in the three tests that performed best compared to the MAb EIA were tested by Western blotting (University of Washington, Seattle). A random sample of concordant samples was also tested, and the sensitivities and specificities of the different tests were calculated, taking into account this sampling strategy. The sensitivities of the tests ranged from 86 to 100%; the specificities ranged from 47 to 99%. The tests that performed best were the Gull Premier EIA (sensitivity, 86.3%; specificity, 97.6%) and the Kalon Biological (sensitivity, 92.3%; specificity, 97.7%) and Biokit (sensitivity, 86.7%; specificity, 92.6%) tests. It cannot be assumed that enzyme immunoassays for the detection of HSV-2 infection that perform well in industrialized countries will perform equally well in other populations.

Item Type: Article
Keywords: Hiv-infection, association, prevalence, behavior, hominis, tests, risk, sera
Faculty and Department: Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
Research Centre: Centre for Global Non-Communicable Diseases (NCDs)
Tropical Epidemiology Group
Centre for Maternal, Reproductive and Child Health (MARCH)
Web of Science ID: 222672100014
URI: http://researchonline.lshtm.ac.uk/id/eprint/14427

Statistics


Download activity - last 12 months
Downloads since deposit
0Downloads
314Hits
Accesses by country - last 12 months
Accesses by referrer - last 12 months
Impact and interest
Additional statistics for this record are available via IRStats2

Actions (login required)

Edit Item Edit Item