A dam mutant of Yersinia pestis is attenuated and induces protection against plague

Robinson, VL; Oyston, PCF; Titball, RW; (2005) A dam mutant of Yersinia pestis is attenuated and induces protection against plague. FEMS microbiology letters, 252 (2). pp. 251-256. ISSN 0378-1097 DOI: https://doi.org/10.1016/j.femsle.2005.09.001

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We have constructed a dam mutant of Yersinia pestis GB. In BALB/c mice inoculated subcutaneously, the median lethal dose of the mutant was at least 2000-fold higher than the wild type. Mice inoculated with sub-lethal doses of the mutant were protected against a subsequent challenge with virulent Y. pestis. The effect of darn inactivation on gene expression was examined using a DNA microarray, which revealed increased expression of a number of genes associated with the SOS response. These results confirm the key role of Dam in the regulation of virulence, and its potential role as a target for the generation of attenuated strains of pathogenic bacteria. Crown Copyright (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies. All rights reserved.

Item Type: Article
Keywords: DNA adenine methylase, plague, vaccine, Dna adenine methylase, escherichia-coli, gene-expression, pseudotuberculosis, methyltransferase
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 16188402
Web of Science ID: 233185700010
URI: http://researchonline.lshtm.ac.uk/id/eprint/12458


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