Grey variants of the live vaccine strain of Francisella tularensis lack lipopolysaccharide O-antigen, show reduced ability to survive in macrophages and do not induce protective immunity in mice


Hartley, G; Taylor, R; Prior, J; Newstead, S; Hitchen, PG; Morris, HR; Dell, A; Titball, RW; (2006) Grey variants of the live vaccine strain of Francisella tularensis lack lipopolysaccharide O-antigen, show reduced ability to survive in macrophages and do not induce protective immunity in mice. Vaccine, 24 (7). pp. 989-996. ISSN 0264-410X DOI: 10.1016/j.vaccine.2005.08.075

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Abstract

Francisella tularensis live vaccine strain (LVS) produces two colony types when grown on solid media, often referred to as blue variants (BV) and grey variants (GV). Whereas blue variant bacteria possessed a lipopolysaccharide O-side chain, grey variant bacteria lacked O-side chains. Grey variant bacteria appeared in stationary phase bacterial cultures and Could be identified using a novel FACS-based assay. Compared to blue variant bacteria, grey variants showed a reduced ability to infect and survive in macrophages. The immunisation of mice with blue variant bacteria, but not grey variant bacteria, induced protective immunity towards fully virulent F tularensis. (c) 2005 Published by Elsevier Ltd.

Item Type: Article
Keywords: vaccine, O-antigen, tularemia, LVS, Tularemia vaccine, lvs, serum, immunogenicity, Animals, Bacterial Vaccines, immunology, Cell Line, Flow Cytometry, Francisella tularensis, classification, immunology, isolation & purification, physiology, Macrophages, microbiology, Mice, Mice, Inbred BALB C, O Antigens, physiology, Research Support, Non-U.S. Gov't
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
PubMed ID: 16257097
Web of Science ID: 235672400019
URI: http://researchonline.lshtm.ac.uk/id/eprint/12002

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