Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand


Na-Ubol, M; Samosornsuk, S; von Seidlein, L; Tapchaisri, P; Ali, M; Clemens, JD; Chaicumpa, W; (2006) Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand. Epidemiology and infection, 134 (5). pp. 997-1003. ISSN 0950-2688 DOI: 10.1017/S0950268806005899

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Abstract

In this study, we used plasmid profile analysis, XbaI macrorestriction with pulsed-field gel electrophoresis (PFGE), and PCR of the ipaH gene, to study the molecular characteristics of 183 Shigella spp. isolated during May 2000 to April 2003 from rectal swabs of patients with watery and/or bloody diarrhoea in a new industrialized area of Thailand. Among the 183 isolates, 167 were S. sonnei and 16 were S. flexneri. For plasmid profile analysis, the 183 isolates revealed 16 different plasmid patterns, designated patterns A to P. The sizes of the plasmid bands were: 6, 5.5, 5, 4.5, 4, 3.25, 2.75, 2.5, 2, 1.75, 1.5 and/or 1.25 kb. The frequency of each plasmid band was 4.5 kb (165 isolates), 3.25 kb (161 isolates), 5.5 kb (129 isolates), 1.75 kb (121 isolates), 1.5 kb (35 isolates), 5 kb (21 isolates), 2 kb (16 isolates), 2.75 kb (12 isolates), 1.25 kb (9 isolates), and 6 kb (8 isolates). PFGE analysis revealed 45 different XbaI macrorestricted DNA banding patterns which could be grouped into 11 groups. All the isolates gave PCR amplicons of the ipaH gene. Plasmid profile analysis and PFGE are powerful tools for differentiation of the Shigella spp. This study provides important data on the molecular characteristics of Shigella isolates in Thailand, which could be useful as an epidemiological baseline for identifying relationships with strains that may emerge in the future.

Item Type: Article
Keywords: *Bacterial Typing Techniques, Diarrhea/epidemiology/*microbiology, Electrophoresis, Gel, Pulsed-Field, Female, Humans, Male, Plasmids, Polymerase Chain Reaction, Shigella/*classification/isolation & purification, Thailand/epidemiology, Urban Population, Bacterial Typing Techniques, Diarrhea, epidemiology, microbiology, Electrophoresis, Gel, Pulsed-Field, Female, Humans, Male, Plasmids, Polymerase Chain Reaction, Shigella, classification, isolation & purification, Thailand, epidemiology, Urban Population
Faculty and Department: Faculty of Infectious and Tropical Diseases > Dept of Disease Control
PubMed ID: 16438746
Web of Science ID: 241138600014
URI: http://researchonline.lshtm.ac.uk/id/eprint/10272

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