Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin.
O'Sullivan, DM;
Hinds, J;
Butcher, PD;
Gillespie, SH;
McHugh, TD;
(2008)
Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin.
The Journal of antimicrobial chemotherapy, 62 (6).
pp. 1199-1202.
ISSN 0305-7453
DOI: https://doi.org/10.1093/jac/dkn387
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OBJECTIVES: To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis. METHODS: Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression. RESULTS: Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment. CONCLUSIONS: The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.
