Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.

Mayta, Holger; Gilman, Robert H; Arenas, Fanny; Valencia, Teresa; Caviedes, Luz; Montenegro, Sonia H; Ticona, Eduardo; Ortiz, Jaime; Chumpitaz, Rosa; Evans, Carlton A; +2 more...Williams, Diana L; and Tuberculosis Working Group in Perú (2003) Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru. Journal of clinical microbiology, 41 (12). pp. 5774-5777. ISSN 0095-1137 DOI: 10.1128/JCM.41.12.5774-5777.2003
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Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.

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