Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.

Mayta, H; Gilman, RH; Arenas, F; Valencia, T; Caviedes, L; Montenegro, SH; Ticona, E; Ortiz, J; Chumpitaz, R; Evans, CA; +2 more...Williams, DL; Tuberculosis Working Group in Perú and (2003) Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru. Journal of clinical microbiology, 41 (12). pp. 5774-5777. ISSN 0095-1137 DOI: 10.1128/JCM.41.12.5774-5777.2003
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Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.

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