Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria.
Samuel Crocodile
Wassmer
;
Georges Emile Raymond
Grau
;
Ian James Callum
MacCormick
;
(2022)
Bridging and Clumping: Investigating Platelet Interactions with P. falciparum-Infected Red Blood Cells and Endothelial Cells in Cerebral Malaria.
In: Jensen, ATR; Hviid, L, (eds.)
Malaria Immunology. Methods in Molecular Biology.
Humana, New York, pp. 505-514.
ISBN 97810716-21899
DOI: 10.1007/978-1-0716-2189-9_38
The methods presented in this chapter describe how to perform ex vivo clumping and in vitro bridging assays in the context of cerebral malaria. Both the protocols are detailed, and emphasis is made on how to prepare platelet suspensions suitable to each technique, including description of specific buffers and reagents to minimize the risk of aggregation while maintaining the platelet properties.
Item Type | Book Section |
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Elements ID | 181756 |
Official URL | http://dx.doi.org/10.1007/978-1-0716-2189-9_38 |
Date Deposited | 01 Aug 2023 11:40 |
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copyright - Available under Copyright the author(s)
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ORCID: https://orcid.org/0000-0002-6753-3376
ORCID: https://orcid.org/0000-0002-0442-0462
ORCID: https://orcid.org/0000-0002-8171-0332