Modified Plasmodium falciparum Ring-Stage Survival Assay with ML10 Kinase Inhibitor.
Platon, L
; Baker, DA; Ménard, D and
(2023)
Modified Plasmodium falciparum Ring-Stage Survival Assay with ML10 Kinase Inhibitor.
Antimicrobial agents and chemotherapy, 67 (5).
e0001723-.
ISSN 0066-4804
DOI: 10.1128/aac.00017-23
; Baker, DA; Ménard, D and
(2023)
Modified Plasmodium falciparum Ring-Stage Survival Assay with ML10 Kinase Inhibitor.
Antimicrobial agents and chemotherapy, 67 (5).
e0001723-.
ISSN 0066-4804
DOI: 10.1128/aac.00017-23
The ring-stage survival assay is the reference assay to measure in vitro Plasmodium falciparum artemisinin partial resistance. The main challenge of the standard protocol is to generate 0-to-3-h postinvasion ring stages (the stage least susceptible to artemisinin) from schizonts obtained by sorbitol treatment and Percoll gradient. We report here a modified protocol facilitating the production of synchronized schizonts when multiple strains are tested simultaneously, by using ML10, a protein kinase inhibitor, that reversibly blocks merozoite egress.
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subject - Accepted Version
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error - This is an author accepted manuscript version of an article accepted for publication, and following peer review. Please be aware that minor differences may exist between this version and the final version if you wish to cite from it.
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