Klebsiella pneumoniae with capsule type K64 is overrepresented among invasive disease in Vietnam
Vu Thi Ngoc, Bich;
Brisse, Sylvain;
Dao Tuyet, Trinh;
Vu Tien Viet, Dung;
Holt, Kathryn E;
Nguyen Vu, Trung;
Tran Thi Kieu, Huong;
Nguyen Thi Ngoc, Diep;
van Doorn, H Rogier;
Wertheim, Heiman FL;
(2021)
Klebsiella pneumoniae with capsule type K64 is overrepresented among invasive disease in Vietnam.
F1000Research, 10.
p. 454.
ISSN 2046-1402
DOI: https://doi.org/10.12688/f1000research.52799.1
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Introduction: Recent reports indicate the emergence of community-acquired pneumonia associated with K64-Klebsiella pneumoniae. Here, we identify the capsular types and sequence type of invasive and commensal K. pneumoniae isolates from Vietnam.
Methods: We included 93 K. pneumoniae isolates from patients hospitalized at the National Hospital for Tropical Diseases, Hanoi between 2007 and 2011; and 110 commensal isolates from throat swabs from healthy volunteers living in rural and urban Hanoi in 2012. We determined sequence types (STs) by multi-locus sequence typing (MLST) and capsule typing for seven K types by PCR. Antibiotic susceptibility testing was performed using disk diffusion.
Results: The most common detected capsule types were K1 (39/203, 19.2%, mainly ST23) and K2 (31/203, 15.3%, multiple STs: ST65, ST86, ST380). We found significantly more K2 isolates among invasive in comparison to commensal isolates (22.6% vs 9%, p = 0.01) but no significant difference was observed between invasive and commensal K1 isolates (14.5% vs 24.7%, p = 0.075). K64 with varying sequence types were predominantly seen among invasive K. pneumoniae (8 vs. 3) and were isolated from sepsis and meningitis patients. Among K64 isolates, one was carbapenem-resistant with ST799.
Conclusion: Our study confirms that capsule type K64 K. pneumoniae is associated with community-acquired invasive infections in Vietnam. Research is needed to unravel the mechanisms of virulence of capsule type K64 in both community and hospital settings.